The binding of [125I]‐angiotensin to rat renal epithelial cell membranes
Open Access
- 1 May 1983
- journal article
- research article
- Published by Wiley in British Journal of Pharmacology
- Vol. 79 (1) , 63-70
- https://doi.org/10.1111/j.1476-5381.1983.tb10496.x
Abstract
Specific high affinity binding sites for [125I]‐angiotensin II have been identified in crude basolateral and brush border membranes from rat renal cortex. A central high affinity site, KD 0.62 nM; Bmax 299 fmol/mg was identified as part of a complex multicomponent binding system. This high affinity site was saturable and exhibited specificity for angiotensin II analogues and closely related peptides but not for bradykinin, substance P or peptide fragments of angiotensin II. Specific [125I]‐angiotensin II binding was partially dependent on NaCl. Absence of NaCl resulted in a decrease in Bmax, had no effect on the rate of association but increased the rate of dissociation of [125I]‐angiotensin from its binding site.This publication has 26 references indexed in Scilit:
- Evidence for mesangial glomerular receptors for angiotensin II linked to mesangial cell contractilityFEBS Letters, 1980
- Regulation of noradrenaline release by presynaptic receptor systemsPublished by Springer Nature ,1977
- High affinity binding of 125I-angiotensin II to rat glomerular basement membranes.Journal of Clinical Investigation, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- RADIOIMMUNOASSAY OF ANGIOTENSIN II IN THE RATJournal of Endocrinology, 1976
- Evidence for renal glomerular receptors for angiotensin IIKidney International, 1974
- Cation Dependence of High-Affinity Angiotensin II Binding to Adrenal Cortex ReceptorsScience, 1974
- STUDIES ON THE ORGANIZATION OF THE BRUSH BORDER IN INTESTINAL EPITHELIAL CELLSThe Journal of cell biology, 1965