Signal transduction alterations in peripheral nerves from streptozotocin‐induced diabetic rats

Publisher Website
Google Scholar
Abstract
We have previously determined the presence of muscarinic receptors and the expression of several G proteins in homogenates and myelin fractions from ra sciatic nerves. In the present study we investigates whether changes in several signal transduction path ways in peripheral nerves might be responsible for some of the biochemical abnormalities (e.g., phosphoinositide metabolism) present in sciatic nerves from streptozotocin‐induced diabetic rats. Sciatic nerves from 5 week diabetic rats that were prelabelled with [3H]‐myo‐inositol displayed a significant increase in the basal release of inositol mono‐and bis‐phosphate, while carbamylcholine‐stimulated release was significantly smaller. Basal‐ and forskolinstimulated adenylyl cyclase activity was significantly decreased in sciatic nerve homogenates from diabetic animals. However, we were unable to detect any significant differences in the levels of cAMP in intact nerves or in nerve segments that were incubated in the presence or absence of forskolin. ADP‐ribosylation experiments showed that in sciatic nerves from experimentally diabetic rats there was a significant increase in the ADP‐ribosylation catalyzed by cholera and pertussis toxins. Measurements of the levels of a‐subunits of G proteins revealed that the expression of Gq/11α, Gsα, and Gi‐3α was increased by 30 to 50%. These results indicate that during the course of experimental diabetes, peripheral nerves exhibit an abnormal production of inositol phosphates and cAMP, together with an abnormal expression and/or function of G proteins. One of the consequences of such alterations is the diminished release of inositol phosphates triggered by muscarinic agonists in diabetic sciatic nerves.