Supercritical Fluid Extraction of Mycotoxins from Feeds with Analysis by Lc/Uv and Lc/Ms
- 1 February 1997
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography & Related Technologies
- Vol. 20 (4) , 537-551
- https://doi.org/10.1080/10826079708010943
Abstract
The use of supercritical fluid extraction with supercritical carbon dioxide and a matrix modifier is described for the isolation of three trichothecene mycotoxins from yellow corn meal and rolled oats. The quantity of supercritical carbon dioxide used in each extraction was 30 mL which contained 5% methanol used as a modifier. The pressure was maintained at 550 atm., and the temperature of the extraction chamber was set at 60 degrees. Recovery of the target analytes from cereal and corn feed by supercritical fluid extraction (SFE) varied from 85 to 95 % depending on the target compound. The mycotoxins, deoxynivalenol (DON), deacetoxyscirpenol (DAS) and T-2 toxin (T-2) were monitored in the SFE extracts by high performance liquid chromatography with UV-detection (HPLC-UV) or ion spray mass spectrometry under full-scan, selected ion monitoring (SIM) and selected reaction monitoring (SRM) modes. There are significant restrictions in the HPLC-UV detection limits for T-2 and DAS, in particular, due to their low UV absorbance. With HPLC-UV and also HPLC-MS in the full-scan mode of acquisition the considerable interference from co-eluting fat-soluble compounds precluded detection of these compounds in feed extracts. In the SIM and SRM LC/MS modes the target analytes could be readily detected at the 250 ppb level in the cereal SFE matrix extracts.Keywords
This publication has 25 references indexed in Scilit:
- Extraction of Airborne Organic Contaminants from Adsorbents Using Supercritical FluidJournal of Chromatographic Science, 1995
- Supercritical Fluid Chromatography and ExtractionAnalytical Chemistry, 1994
- Analytical-scale supercritical fluid extraction: a promising technique for the determination of pollutants in environmental matricesJournal of Chromatography A, 1993
- Factors controlling quantitative supercritical fluid extraction of environmental samplesJournal of Chromatography A, 1993
- Comparison of hydrodistillation and supercritical fluid extraction for the determination of essential oils in aromatic plantsJournal of Chromatography A, 1993
- Sample preparation by supercritical fluid extraction in environmental food and polymer analysisChromatographia, 1991
- Separation and Identification of Mycotoxins by Thin-Layer Chromatography/Fast Atom Bombardment Mass Spectrometry.Analytical Sciences, 1991
- Supercritical fluid extraction and chromatography of cholesterol in food samplesJournal of Chromatography A, 1990
- Extraction of fat tissue from meat products with supercritical carbon dioxideJournal of Agricultural and Food Chemistry, 1989
- Fluorodensitometric determination of trichothecene mycotoxins with nicotinamide and 2-acetylpyridine on a silica gel layerJournal of Chromatography A, 1982