CHARACTERISTICS OF 19S AND 7S RESPONSE TO BACTERIOPHAGE OX174 IN FOWL

  • 1 January 1966
    • journal article
    • research article
    • Vol. 10  (2) , 169-+
Abstract
After intravenous injection of 1010 plaque forming particles of OX174 bacteriophage into White Rock fowls, immune elimination began at 30 hours and viable phage was cleared from the circulation by 50-52 hours, the approximate time at which detectable antibody appeared. Little change was noted in the serum neutralizing activity in the interval from 44 to 217 days after injection, at which time the birds were reinjected with OX174. Sephadex Peak I (19S globulin) accounted for most of the early activity of the primary response. By Day 9 most of the activity had shifted to Peak II (7S globulin). In the secondary response, the shift had occurred by Day 4. Both 19S and 7S globulin fractions showed an increase in activity when compared to the same days of the primary response, but the 7S increase was proportionately greater. All sera and serum fractions (whether 19S or 7S) were sensitive to reduction with 2-mercaptoethanol, but became less sensitive during the course of immunization. With regard to the production of 19S antibody, White Rock fowls showed a different response to the particulate antigen OX174 compared with the response to injection of the soluble antigen, bovine serum albumin. There is evidence of immunological memory in the 19S response to OX174.