The B–Z transition in two synthetic oligonucleotides: d(C-2-amino-ACGTG) and d(m5CGCAm5CGTGCG) studied by IR, NMR and CD spectroscopies
- 10 August 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (15) , 6291-6305
- https://doi.org/10.1093/nar/12.15.6291
Abstract
The sequences CA'CGTG (where A'=2-aminodeoxyadenosine) and m5CGCAm5CGTGCG are prepared and studied by IR, CD and 1H-NMR. Infrared spectra demonstrate the capacity of the modified hexamer and decamer to adopt a Z conformation. The influence of the NH2 substitution on the adenine or of the methylated terminal part of the decamer acting with the increase of the DNA concentration stabilizes the Z conformation at room temperature in low humidity films. Very weak proportion of Z conformation is detected in UV dilute solutions. In more concentrated NMR solutions, the Z proportion induced by high salt content is only 20–25%. The effects of the concentration and of the covalent modification of the bases are discussed.Keywords
This publication has 22 references indexed in Scilit:
- Proton NMR and circular dichroism studies of the B and Z conformations of the self-complementary deoxyhexanucleotide d(m5C-G-C-G-m5C-G): mechanism of the Z-B-coil transitionsBiochemistry, 1984
- Poly(d2NH2A-dT): Effect of 2-amino substituent on the B to Z transitionBiochemical and Biophysical Research Communications, 1984
- Caractérisation de l'activité l-thrénine désaminasique du foie de cobaye induite par un régime hyperprotidique.Biochimie, 1984
- Left-handed helical conformation of poly[d(A-m5C) · d(G-T)]Nature, 1983
- DNA Fragment ConformationsEuropean Journal of Biochemistry, 1983
- Structural transitions in DNAs and nucleohistones studied by I.R. spectroscopyBiochimie, 1982
- Conformation and dynamics in a Z-DNA tetramerJournal of Molecular Biology, 1981
- High-salt d(CpGpCpG), a left-handed Z′ DNA double helixNature, 1980
- 2-Aminoadenine is an adenine substituting for a base in S-2L cyanophage DNANature, 1977
- Salt-induced co-operative conformational change of a synthetic DNA: Equilibrium and kinetic studies with poly(dG-dC)Journal of Molecular Biology, 1972