Factors influencing bacterial deamination

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Abstract
Dl-serine is deaminated by washed suspensions aerobically and anaerobically with the high QN of 200-1000; the activity is decreased 95% by 2% glucose in the growth medium. The greatest activity is obtained in cultures 11 hrs. old, and then falls off. Washed suspensions lose activity on standing in water at 0[degree] or at 37[degree], aerobically or anaerobically. Loss of activity is prevented by boiled suspensions of organisms and by diffusate from them; by M/100 phosphate and by various reducing systems (GSH, H2 and formate). Activity is restored by boiled bacteria, M/30,000 GSH, and M/200 formate; all these require phosphate. If decay of activity has occurred aerobically phosphate alone is ineffective in producing recovery. If loss of activity has occurred anaerobically a slow incomplete recovery occurs with phosphate alone probably acting in conjunction with some residual reducing system in the cell.

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