Low-Temperature Storage as a Quarantine Treatment for the Caribbean Fruit Fly (Diptera: Tephritidae) in Florida Citrus

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Abstract
‘Marsh’ and ‘Ruby Red’ grapefruits were infested by the Caribbean fruit fly, Anastrepha suspensa (Loew), in a cage, held at 10 or 15.6°C for 7 days (cold conditioning), then stored at 1.7°C for periods of 7, 9, 12, 15, and 19 days. ‘Pineapple’ and ‘Valencia’ oranges were handled in the same way except they were not “conditioned” before cold storage. The exposure periods required to provide 99.9968% mortality (probit 9) of A. suspensa infestations in grapefruit, based on puparia recovered, were calculated by computer-programmed probit analysis to be 30 days for fruit conditioned at 10°C and 18.6 days for fruit conditioned at 15.6°C No A. suspensa survived in ‘Pineapple’ oranges exposed to 1.70°C for 12, 15, or 19 days. Attempts to infest ‘Valencia’ oranges with the Caribbean fruit fly were unsuccessful.