Acid Mucopolysaccharide Determination: A Useful Adjunct for the Diagnosis of Malignant Mesothelioma with Effusion
- 1 September 1969
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 52 (3) , 335-339
- https://doi.org/10.1093/ajcp/52.3.335
Abstract
Acid mucopolysaccharide (AMP) content in pleural and peritoneal fluid is quantified by precipitating the AMP with acid ethanol, solubilized in a dilute NaOH solution, and reacting the hexuronic acid with the carbazole reagent. An Allen correction in the final reading step eliminates the effects of interfering substances. This simplified analysis for AMP, by only partially purifying the AMP prior to color development, has been found suitable for use in the clinical laboratory. Studies on effusions from 11 patients have shown no detectable AMP by this method in seven patients without mesotheliomas and significant elevations of AMP in all three patients with untreated mesotheliomas. One patient with mesothelioma, previously treated with chemotherapy, had no detectable levels on two separate occasions. The determination of AMP in pleural and peritonea] fluid is a useful and practical adjunct to the clinical diagnosis of mesothelioma.Keywords
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