Conformational studies on gastrin related peptides by high resolution 1H‐n.m.r.
- 1 February 1986
- journal article
- research article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 27 (2) , 145-152
- https://doi.org/10.1111/j.1399-3011.1986.tb01804.x
Abstract
The secondary structures of three gastrin analogs, HCl · H‐Trp‐Nle‐Asp(O‐tBu)‐Phe‐NH2 (tetragastrin), pGlu‐Ala‐Tyr‐Gly‐Trp‐Nle‐Asp‐Phe‐NH2 (octagastrin), and H‐Leu‐(Glu)5‐Ala‐Tyr‐Gly‐Trp‐Nle‐Asp‐Phe‐NH2 (minigastrin) were studied by 1H‐n.m.r. in dimethylsulfoxide and in trifluoroethanol. All three compounds were found to assume a random conformation in the former solvent, while some ordered secondary structure is present in trifluoroethanol even at the tetra‐peptide level. This was shown by temperature studies and solvent titrations. At least four amide protons were found to be solvent shielded in the longer hormone.Keywords
This publication has 12 references indexed in Scilit:
- Conformational properties of gastrin fragments of increasing chain lengthBiopolymers, 1985
- Synthese von Gastrin-aktiven Peptiden. Untersuchungen zur Struktur-Wirkungsbeziehung des natürlichen Hormons Human-Little-Gastrin-IHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1984
- Zur Synthese von Human-Little-Gastrin-I und dessen Leucin-15-, Norleucin-15- und Methoxinin-15-AnalogaHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1983
- Lipid-induced ordered conformation of some peptide hormones and bioactive oligopeptides: predominance of the helix over the .beta.-formBiochemistry, 1982
- Conformational aspects of gastrin‐related peptides: A circular dichroism studyBiopolymers, 1981
- CONFORMATIONAL CALCULATIONS ON GASTRIN C‐TERMINAL TETRAPEPTIDEInternational Journal of Peptide and Protein Research, 1981
- A review of some recent developments in the chemistry of the gastrinsBioorganic Chemistry, 1979
- Minigastrin: Corrected Structure and SynthesisHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1979
- Pure Human Big Gastrin IMMUNOCHEMICAL PROPERTIES, DISAPPEARANCE HALF TIME, AND ACID-STIMULATING ACTION IN DOGSJournal of Clinical Investigation, 1974
- Conformational study of some component peptides of pentagastrinJournal of the Chemical Society, Perkin Transactions 2, 1972