Improved Solid Phase Extraction Procedure for Assay of Cephalosporins in Human Urine Samples

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Abstract
Solid phase extraction technique has been evaluated for the treatment of urine samples in the analysis of cephalosporins before injection into an HP-Hypersil ODS-C18 column. Cephalexin, cefotaxime, cefazolin, cefuroxime, and cefoxitin were tested with seven different reversed-phase extraction column cartridges and the obtained urine extracts were not clean. However, 3M Empore extraction disk cartridges packed with octadecyl (C18) bonded silica provided clean extracts with a single extraction. The recoveriés of the five cephalosporins ranged from 56 to 60 % in the 1.25–500 g/mL concentration range. The assay was accurate, precise, and adequate for testing the drug content in urine samples. The detection limit found for the five drugs was 0.25 g/mL after an injection of 10 L. The reliability of this study was tested by analyzing a urine sample containing cefuroxime after a minimum single dose administration (250 mg).