A new technique for in vivo percutaneous absorption.

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Abstract
One method to evaluate the amount of a drug that is percutaneously absorbed is to recover and measure the amount of the drug that remains on the skin. Wiping the skin with cotton soaked in an ointment base is the method ordinarily used to recover the unabsorbed drug. The recovery rate with this method varies widely depending on the ointment base. In general, it is difficult to quantitatively remove the drug vehicle and the drug from the skin except with certain vehicles such as water. In order to improve the method of drug recovery, a simple and reproducible in vivo method using rats was developed. The test drug was applied within an overflow barrier designed to maintain a constant drug application area, and five hours later the skin was excised without recovering the applied ointment and the level of residual drug determined. The recovery rate was nearly 100% when the skin was totally excised without wiping immediately after drug application. The overflow of drug from the ointment base was prevented by the overflow barrier, and the application area was kept constant. This method should be useful to evaluate percutaneous absorption of drugs quantitatively.

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