Effect of abscisic acid on membrane-bound epidermal ATPase from tobacco leaves
- 1 April 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 20 (2) , 293-300
- https://doi.org/10.1093/oxfordjournals.pcp.a075813
Abstract
Membrane-bound Mg++-activated ATPase activity in epidermal strips from tobacco leaves (Nicotiana tabacum L. Samsun NN) was stimulated by abscisic acid (ABA) when the strips were floated on ABA solution in light or in darkness. The optimum ABA concentrations in light and in darkness were 10−5 M and 10−6 M, respectively. Carbonyl cyanide m-chlorophenylhydrazone (CCCP) and N, N′-dicyclohexylcarbodiimide (DCCD) completely blocked the basal level membrane-bound epidermal ATPase activity. ABAinduced membrane-bound epidermal ATPase activity was completely inhibited by CCCP, but only partly by DCCD. H+-influx into epidermal strips on a solution in light was lower than that in darkness. ABA stimulated H+-influx into epidermal strips in light and in darkness. CCCP suppressed basal level H+-influx, whereas DCCD did not. CCCP also suppressed ABA-induced H+-influx, whereas DCCD did not. Interaction between H+-influx and membranebound epidermal ATPase activity is discussed.This publication has 8 references indexed in Scilit:
- Characterization of membrane-bound Mg++-activated ATPase isolated from the lower epidermis of tobacco leavesPlant and Cell Physiology, 1979
- Response of Membrane-bound Mg2+-activated ATPase of Tobacco Leaves to Tobacco Mosaic VirusPlant Physiology, 1978
- Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissueBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Adenosine triphosphatase in epidermal tissue of Commelina benghalensis: Possible involvement of isozymes in stomatal movementPlant Science Letters, 1976
- Cation-stimulated Adenosine Triphosphatase Activity and Cation Transport in Corn RootsPlant Physiology, 1976
- Auxin-gibberellin relationships in. their effects on hypocotyl elongation of light-grown cucumber seedlings IV. Inhibition of IAA-induced elongation by N,N′-dicyclohexylcarbodiimide and its reversal by gibberellin A3Plant and Cell Physiology, 1976
- In vitro binding of IAA to plasma membrane-rich fractions containing Mg++-activated ATPase from mung bean hypocotylsPlant and Cell Physiology, 1976
- Auxin-induced hydrogen ion excretion: correlation with growth, and control by external pH and water stressPlanta, 1975