Recombinant human extracellular superoxide dismutase reduces concentration of oxygen free radicals in the reperfused rat heart

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Abstract
Study objective – The aim of the study was to determine whether recombinant human extracellular superoxide dismutase type C (rh-EC-SOD C) has antioxidant effects similar to those of the previously studied Cu-Zn superoxide dismutase. Design – Electron paramagnetic resonance spectroscopy was used for quantification of free radical generation during reperfusion of postischaemic isolated rat hearts. The hearts were perfused with Krebs-Henseleit buffer and then subjected to 15 min global ischaemia. N-tert-butyl-α-phenylnitrone (PBN) was added as a spin trapping agent to the perfusate. Experimental material – 18 hearts from Sprague-Dawley rats (280-400 g) were used. Measurements and main results – Reperfusion resulted in a burst of radical formation. In presence of rh-EC-SOD C plus catalase, the PBN spin adduct concentration was only 18% (pv rh-EC-SOD C). Conclusions – In isolated rat heart, rh-EC-SOD C reduces free radical concentrations at least to the same extent as Cu-Zn superoxide dismutase.

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