Trans-N-glycosidase studied with radioactive adenine

Publisher Website
Google Scholar
Abstract
The exchange between adenine and the hypoxanthine of hypoxanthine deoxyriboside catalysed by an enzyme prepn. from Lactobacillus helveticus was studied. Using C14- adenine the reaction gave rise to adenine deoxyriboside with a molar C14 concn. as high as that of the initial adenine. The reaction was therefore, not a transamination involving the 6-positions of adenine and hypoxanthine deoxyriboside, but was probably an exchange of the adenine skeleton with the bound hypoxanthine, catalysed by a trans-glycosidase. The enzyme prepn. catalysed an exchange reaction between the adenine of adenine deoxyriboside and C14-adenine. It also rapidly degraded adenine riboside (adenosine) by hydrolysis and deamination, but did not deaminate adenine deoxyriboside.