Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus
- 15 June 1977
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 164 (3) , 653-658
- https://doi.org/10.1042/bj1640653
Abstract
The biosynthesis of chitin was obtained in broken mycelia and protoplasts of the fungus A. fumigatus. The specific activity of chitin synthase (EC 2.4.1.16) in a membrane preparation from protoplasts derived from the hyphal tips of A. fumigatus was 26.8-fold greater than that of the chitin synthase in broken mycelia, indicating that the active chitin synthase is located primarily in a membrane-bound site at the hyphal tip. Polyoxin D was a potent competitive inhibitor of the enzyme, having Ki 5.2 .+-. 0.8 .mu.M with respect to the natural substrate UDP-N-acetyl-D-glucosamine, which has Km 1.58 mM.This publication has 17 references indexed in Scilit:
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