Use of somaclonal variation in the improvement of Eustoma grandiflorum

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Abstract
Approximately 750 plants were regenerated from stem, leaf, and meristem explants of Eustoma grandiflorum. Most of the regenerants showed phenotypic characteristics of a single stem with branching in the mid-portion to the top of the plant. A strong basal branched characteristic, present in 10 plants, was not present in the original seedling population selected for tissue culture. The frequency of genetically branched somaclones was 1.3 percent. Basal branched somaclones produced more flowers than the single stemmed parent cultivar.

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