Analysis of RNase A refolding intermediates by electrospray/mass spectrometry
- 3 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (3) , 301-306
- https://doi.org/10.1016/0014-5793(94)00966-x
Abstract
Electrospray/mass spectrometry (ESIMS) was extensively used to obtain information on disulphide-containing intermediates formed during refolding of bovine pancreatic ribonuclease A. The analysis showed the existence of an equilibrated population of disulphide bonded intermediates, and indicates that intermediates containing two intramolecular S–S are predominant until late stages of the refolding process. Mixed disulphides with exogenous glutathione were also detected, supporting previous evidence of conformational restrictions on the ability of RNase A to form intramolecular disulphides. The results indicate that ES/MS is a suitable technique to detect and characterize refolding intermediates.Keywords
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