Measurement of Urinary 7,8-Dihydro-6-hydroxylumazine in Healthy and in Ehrlich Ascites Tumour-Bearing Mice
- 1 January 1984
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 365 (2) , 895-900
- https://doi.org/10.1515/bchm2.1984.365.2.895
Abstract
An estimation of 7,8-dihydro-6-hydroxylumazine in urinary samples of mice by high performance liquid chromatography on reversed phase material is presented. Urinary samples are diluted with water and oxidized by iodine. Chromatographic elution is done isocratically using aqueous buffer containing methanol. Effluent 6-hydroxylumazine is monitored by its fluorescence (382 nm excitation, 449 nm emission). The described method can be performed rapidly with satisfactory accuracy and precision. The electrochemical detection of 7,8-dihydro-6-hydroxylumazine was studied additionally. Total values of daily excreted 7,8-dihydro-6-hydroxylumazine of healthy and of Ehrlich ascites tumor-bearing mice were measured.This publication has 1 reference indexed in Scilit: