Abstract
Carboxymethylation of Hb at pH 9 (optimum pH) took place in 60% of histidine and 2-3% of tyrosine residues in 10 hours at 30[degree]. Pretreatment of Hb with 2-8 M urea increased carboxymethylation of histidine and tyrosine groups and caused a marked shift of Soret band.