Taxonomic significance of protein patterns of rust species and formae speciales obtained by disc electrophoresis

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Abstract
Soluble uredospore proteins were isolated from physiologic races or isolates of Puccinia graminis f. sp. avenae, P. graminis f. sp. secalis, P. graminis f. sp. tritici, P. recondita f. sp. secalis, P. recondita f. sp. tritici, P. coronata f. sp. avenae, and P. hordei. The protein extracts were subjected to polyacrylamide gel (disc) electrophoresis and the resulting patterns were compared.Patterns were assessed by two methods based on Ef values and by a third method based on subjective assessment of color intensity of stained proteins. Assessments based on Ef values differed for each method and were in almost total disagreement with conclusions derived by the third method.No useful inter-subspecific and interspecific comparisons were possible using Ef-based data because the host on which the rust was grown influenced the patterns. Evidence obtained by the third method of assessment contradicted this conclusion, for the host only appeared to influence color intensity and width of individual bands. Thus, using the color intensity method it was found that the differences between species were greater than between formae speciales, and the latter, with one exception, differed more than physiologic races. Differentiation between most physiologic races or isolates was impossible.The taxonomic significance of the protein patterns is discussed.

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