Up-regulation of NAD(P)H quinone oxidoreductase 1 during human liver injury
Open Access
- 1 January 2006
- journal article
- Published by Baishideng Publishing Group Inc. in World Journal of Gastroenterology
- Vol. 12 (12) , 1937-1940
- https://doi.org/10.3748/wjg.v12.i12.1937
Abstract
AIM: To investigate the expression and activity of NAD(P)H quinone oxidoreductase 1 (NQO1) in human liver specimens obtained from patients with liver damage due to acetaminophen (APAP) overdose or primary biliary cirrhosis (PBC). METHODS: NQO1 activity was determined in cytosol from normal, APAP and PBC liver specimens. Western blot and immunohistochemical staining were used to determine patterns of NQO1 expression using a specific antibody against NQO1. RESULTS: NQO1 protein was very low in normal human livers. In both APAP and PBC livers, there was strong induction of NQO1 protein levels on Western blot. Correspondingly, significant up-regulation of enzyme activity (16- and 22-fold, P < 0.05) was also observed in APAP and PBC livers, respectively. Immunohistochemical analysis highlighted injury-specific patterns of NQO1 staining in both APAP and PBC livers. CONCLUSION: These data demonstrate that NQO1 protein and activity are markedly induced in human livers during both APAP overdose and PBC. Up-regulation of this cytoprotective enzyme may represent an adaptive stress response to limit further disease progression by detoxifying reactive species.Keywords
This publication has 21 references indexed in Scilit:
- Differential Expression of Mouse Hepatic Transporter Genes in Response to Acetaminophen and Carbon TetrachlorideToxicological Sciences, 2004
- Bromobenzene-Induced Hepatotoxicity at the Transcriptome LevelToxicological Sciences, 2004
- Molecular signature in three types of hepatocellular carcinoma with different viral origin by oligonucleotide microarray.International Journal of Oncology, 2004
- Acute Liver Failure in the United StatesSeminars in Liver Disease, 2003
- Differential Gene Expression ofNAD(P)H:Quinone OxidoreductaseandNRH:Quinone Oxidoreductasein Human Hepatocellular and Biliary TissueMolecular Pharmacology, 2002
- NAD(P)H:quinone oxidoreductase 1 (NQO1): chemoprotection, bioactivation, gene regulation and genetic polymorphismsPublished by Elsevier ,2001
- Immunodetection of NAD(P)H:quinone oxidoreductase 1 (NQO1) in human tissues11This work is dedicated to the memory of Professor Lars Ernster, who provided us with enthusiastic support, scientific insight, and constant encouragement in our many interactions.Free Radical Biology & Medicine, 2000
- High levels of expression of the NAD(P)H:Quinone oxidoreductase (NQO1) gene in tumor cells compared to normal cells of the same originBiochemical Pharmacology, 1991
- Increase of NAD(P)H:quinone reductase by dietary antioxidants: possible role in protection against carcinogenesis and toxicity.Proceedings of the National Academy of Sciences, 1980
- [56] DT diaphorasePublished by Elsevier ,1967