Increased muscarinic receptor binding in heart membranes by an inhibitor of protein kinase C
- 19 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 242 (1) , 175-177
- https://doi.org/10.1016/0014-5793(88)81010-x
Abstract
The number of muscarinic acetylcholine receptors (MAChRs), as detected by binding of [3H]quinuclidinyl benzilate (QNB), was investigated under conditions which promote protein phosphorylation. Incubation of a crude heart membrane preparation in the presence of ATP/Mg2+ reduced MAChR number by 50%. Incubation with polymyxin B, an inhibitor of protein kinase C, blocked the efrect of ATP/Mg2+ and increased MAChR number by 74%.Keywords
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