Complex structural behavior of oligopurine-ligopyrimidine sequence cloned within the supercoiled plasmid
- 31 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (2) , 617-629
- https://doi.org/10.1093/nar/17.2.617
Abstract
Synthetic sequence GATCC(AG)7ATCG(AT)4CG(AG)7 was cloned into plasmid and its structural behavior under the infuence of supercoiling was analysed by chemical modification at variety of experimental conditions. It was found that this sequence adopts at least two different non-B conformations depending on -.hivin..sigma. and pH values. Moreover, 12 nucleotide long non-pur .cntdot. pyr spacer region separating two identical (AG)7 blocks does not provide a significant energy barrier protecting against unusual structures formation.This publication has 38 references indexed in Scilit:
- Single Strands, Triple Strands, and Kinks in H-DNAScience, 1988
- The S1-sensitive Form of D(C-T)
n
⋅d(A-G)
n
: Chemical Evidence for a Three-Stranded Structure in PlasmidsScience, 1988
- Chemical probing of the homopurine·homopyrimidine tract in supercoiled DNA at single‐nucleotide resolutionFEBS Letters, 1988
- Chemical probing of homopurine-homopyrimidine mirror repeats in supercoiled DNANature, 1988
- A structural basis for S1 nuclease sensitivity of double-stranded DNACell, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Conformation of promoter DNA: Fine mapping of S1-hypersensitive sitesCell, 1983
- Association of an S1 nuclease-sensitive structure with short direct repeats 5′ of Drosophila heat shock genesNature, 1983
- Homocopolymer sequences in the spacer of a sea urchin histone gene repeat are sensitive to S1 nucleaseNature, 1982
- DNA Sequences Mediating Class Switching in α-ImmunoglobulinsScience, 1980