Requirement of B Lymphocytes in Local Adoptive Transfer of Experimental Allergic Orchitis (EAO) by Lymph Node Cells

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Abstract
The necessity of T lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) is an established fact. The present study was undertaken to elucidate the participation of B lymphocytes in the local transfer system of EAO. The capacity of purified lymph node T and B cells to transfer EAO was compared. Donor strain 13 guinea pigs were sensitized with testicular antigen and complete Freund''s adjuvant. Cells (1 .times. 108) were injected just under the tunica albuginea of the testis of a syngeneic recipient. The recipients were killed 5 days after cell transfer. Nylon-wool nonadherent T cells alone produced only minimal lesions, but could induce more severe mononuclear lesions with the addition of syngeneic, normal macrophages. In contrast, B-cell enriched populations, alone or combined with a small number of macrophages, were able to transfer extensive lesions. In the combined transfer of immune T and immune B cells the severity of transferred lesions was dependent upon the number of B cells. Cooperative interactions among T cells, B cells and macrophages are apparently involved in the generation of transferred testicular lesions, with emphasis on the participation of B cells.

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