Identification of 108 K, 93 K, and 42 K glycoproteins of bovine herpesvirus-1 by monoclonal antibodies

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Abstract
Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins g I, g III, and g IV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by immunization of mice with either BHV-1 envelope or virus infected cells, from which the glycoproteins g I, g III, and g IV were removed by immunoaffinity. In the latter immunization procedure mice were tolerized either against normal cellular antigens with or without glycoproteins g I, g III, g IV, and nucleocapsid. From 154 anti-BHV-1 hybridomas isolated, 39 MAbs precipitated a 108 K glycoprotein. Two other glycoproteins of respectively 42 K and 93 K were precipitated each by one MAb. These three glycoproteins were detected in infected cell lysate. Nine anti-108 K glycoprotein MAbs neutralized BHV-1 infectivity and three non-neutralizing MAbs were able to reduce plaque development when virus was grown in the presence of these MAbs. It is therefore suggested that this glycoprotein is involved in viral entry into the cell and in cell-to-cell spread of the virus.

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