Enhancement of Encephalomyocarditis Virus Replication in L Cells Treated with Insulin

Abstract
Treatment of (mouse fibroblast) L cells with low levels of insulin results in a marked increase in the yield of encephalomyocarditis virus, strain MM. Maximum yields were obtained when the cell cultures were pretreated with the hormone for 24 h followed by the presence of insulin after virus infection. The increased virus yields cannot be attributed to increased cell proliferation or to interference with the interferon system.