Double fluorescent labeling method used for a study on liposomes.

1 January 1991

journal article
Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN

Vol. 39 (1) , 227-229
https://doi.org/10.1248/cpb.39.227

Abstract

Carboxyfluorescein (CF) and Calcein (CAL) have almost the same fluorescent spectrum. However, only CAL forms a nonfluorescent chelate with the Co2+ ion. It was proven that this phenomenon can be used for the fractional determination of CF and CAL. And this fractional determination method was available for a study on liposomes.

Keywords

CHELATE
CARBOXYFLUORESCEIN
CALCEIN
DOUBLE
NONFLUORESCENT
LABELING METHOD USED
FLUORESCENT LABELING METHOD

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