Specific assays of hemostasis proteins: Fibrinogen

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Abstract
Summary Fibrinogen levels are considered a useful indicator in several pathological conditions and recent epidemiological studies have indicated a relationship between fibrinogen levels and increased risk of cardiovascular disease. An accurate measurement of this protein is therefore recommended and the Italian Committee for Standardization of Methods in Hematology and Laboratory has carried out a collaborative study to determine accuracy, precision and comparability of results obtained by six different methods, i.e.,1. Blombäck and Blombäck method,2. clotting assay according to von Clauss,3. radial immunodiffusion according to Mancini et al.,4. total amount of clottable fibrinogen by means of turbidimetric assay according to Ellis and Stransky, and5. with ChromotimeSystem,6. prothrombin time (PT)-derived fibrinogen assay on ACL coagulometer. The most accurate resulted the von Clauss method, but only if calibrated with an internal standard; in fact, when the manufacturer’s tables are used, the method proved to be highly inaccurate. The best precision, both intra- and between-laboratory, was obtained by the PT-derived test on ACL. On the basis of this still incomplete evaluation of the CISMEL study data, we can conclude that:i. some methods used in clinical laboratories give accurate results only after adequate calibration;ii. a reference standard pool may be a valid tool for calibration and for a better between-laboratory comparability;iii. a predilution of the samples with high fibrinogen levels seems indicated;iv. automation markedly increases the precision of methods.