Cell cycle related change of Ara‐C transport in HL‐60 cells after differentiation induction

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Abstract
Using a promyelocytic leukemia cell line, HL‐60, we studied the membrane transport of Ara‐C before and after differentiation induced by retinoic acid (RA). In RA‐treated cells, Ara‐C transport was reduced and there was a concomitant increase of the ID50 values of Ara‐C in comparison with the controls. By three different procedures to synchronize untreated cells, i.e. density arrest G1 phase enrichment, aphidicolin‐induced S phase accumulation and the double isoleucine block method, we found that Ara‐C transport was 30–50% higher in the S phase than in the G1 phase. Therefore, the observed decrease in Ara‐C transport is, in part, due to the retarded growth accompanied by an accumulation of cells in the G1 phase after differentiation induction.