Estimation of oestrone sulphate in the serum of pregnant sows

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Abstract
Estrone sulfate was extracted from pig serum with tetrahydrofuran and then hydrolysed with sulfatase. The radioimmunoassay of liberated estrone was carried out directly on the reaction mixture. Estrone sulfate concentrations were < 60 pg/ml in non-pregnant sows. Detectable concentrations of estrone sulfate were found in serum as early as the 18th gestation day, and these rose to maximum levels of 2.1-3.5 ng/ml by days 26-29 then fell to about 0.1 ng/ml by day 37. Serum estrone sulfate concentrations were not related to litter size, but were good indicators of early pregnancy.