Sensitive Methods for the Detection and Characterization of Platelet Isoantibodies

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Abstract
Two sensitive methods for the detection and characterization of platelet isoantibodies are presented in this paper. The methods involve incubating platelets with either radioactive serotonin or adenine, interaction of the platelets with serum antibody and finally detection of supernatant radioactivity. Both tests can be used for HL-A typing of platelets and both tests detect antibodies undetectable by complement fixation. The requirement of a heat labile serum component is variable in the serotonin test but absolute in the adenine test. Antibody activity was found to be in the 7S gamma globulin fraction on DEAE cellulose column chromatography. * Current address: Children's Cancer R esearch Foundation, Boston, Massachusetts.

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