The Primary Structure of the Hemoglobins of the Adult Jaguar(Panthera onco,Carnivora)

Abstract
The primary structure of the hemoglobins from jaguar (Panthera onco) are presented. Electrophoretic separations without and with a dissociating agent revealed the presence of two hemoglobin components, .alpha.2.beta.2I and .alpha.2.beta.2II. The separation of the hemoglobin components was achieved by ion-exchange chromatography. The globin chains were separated by ion-exchange chromatography and also by reversed phase HPLC. The amino-acid sequences of the native chains and peptides were determined by liquid-phase and gas-phase sequencing. N-Acetylserine was detected by FAB-mass spectroscopy as N-terminal group of the .beta.I chain. The sequences are compared with that of human hemoglobin (Hb A).

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