Histochemical Detection of Acid and Alkaline Phospliatases in Periodontal Structures of Rat and Mouse

Abstract
Acid and alkaline phosphatase activities in periodontal structures of normal adult rats and mice were studied. Ethylenediaminetetraacetate (EDTA) neutral solution for decalcification and the simultaneous azo coupling method was used for histochemical detection. The acid phosphatase reaction was usually confined to the superficial layer in the attached gingival epithelium and epithelial attachment. Alkaline phosphatase activity was absent in the gingival epithelium. The periodontal membrane contained an abundant acid phosphatase activity and a slight alkaline phosphatase activity. Acid phosphatase activity was slight in or around the proliferating cementum and alveolar bone.

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