Immunotoxins containing saporin 6 and monoclonal antibodies recognizing plasma cell‐associated antigens: Effects on target cells and on normal myeloid precursors (CFU‐GM)

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Abstract
Monoclonal antibodies 8A and 62B1, recognizing plasma cell‐associated antigens, were covalently linked to saporin 6, a ribosome‐inactivating protein similar to the A‐chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non‐target K562 cell line and on myeloid CFU‐GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A‐saporin 6 and 62B1‐saporin 6 immunotoxins, with an ID50 up to 200000‐fold lower than free saporin 6, whilst the K562 non‐target cell line was unaffected. Normal human myeloid precursors (CFU‐GM) were inhibited by immunotoxins only to a limited extent. An application of this model for autologous bone marrow transplantation in multiple myeloma patients is proposed. Since no eradication of cloning target cells was achieved by a single immunotoxin, mixtures made with different antibodies could help to reach this goal.

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