BIOGENESIS OF RIBOFLAVIN IN GREEN LEAVES

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Abstract
The stoichiometry of the enzymatic formation af riboflavin and 6-methyl-8-ribityI-2,4,7-trioxo-hexahydropteridine from 6,7-dimethyl-8-ribityllumazine was examined with a crude enzyme prepraation obtained from the spinach. One mole of 6-methyl-8-ribityl-2,4,7-trioxo-hexahydropteridine was produced from two moles of 6,7-dimethyl-8-ribityllumazine. The molar ratio of the riboflavin enzymatically produced to 6-methyl-8-ribityl-2,4,7-trioxo-hexahydropteridine produced varied in the presence of cysteine. Formation of 6-methyl-8-ribityl-2,4,7-trioxo-hexahydropteridine from 6,7-dimethyl-8-ribityllumazine was inhibited by ascorbic acid. Pyruvate was incorporated enzymatically neither into riboflavin nor into 6-methyl-8-ribityl-2,4,7-trioxo-hexahy-dropteridine.

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