STUDIES ON IN VITRO ANTIBODY PRODUCTION: II. THE EFFECT OF NEWCASTLE DISEASE VIRUS ON ANTIBODY SYNTHESIS

Publisher Website
Google Scholar
Abstract
Antibody production by spleen cells in vitro permits the study of a unique property of cells, the synthesis of a specific, quantitatively detectable protein. Under certain conditions allantoic fluid containing Newcastle disease virus (NDV), as well as centrifugally purified NDV, when added to a suspension culture of spleen cells from previously immunized rabbits, produced detectable changes in the amount of radioactively labelled antibody produced. NDV also inhibited glycine-C14 incorporation into the TCA-insoluble protein fraction of spleen cells.This inhibition was parallel to the loss of cell viability. No detectable increase of infectious virus or haemagglutination titer was noted. It is suggested that the inhibitions shown were due to the cytotoxic effect of the virus, which appeared to require the infectious virus particle.