The Salm-gene project - a European collaboration for DNA fingerprinting for food-related salmonellosis
Open Access
- 1 February 2003
- journal article
- Published by European Centre for Disease Control and Prevention (ECDC) in Eurosurveillance
- Vol. 8 (2) , 46-50
- https://doi.org/10.2807/esm.08.02.00401-en
Abstract
An external quality assessment of PFGE method to discriminate between salmonella serotypes and lysotypes was carried out by the Salm-Gene project in Europe. A set of 16 strains of S. Enteritidis was sent to 9 national salmonella reference laboratories. By using a harmonised protocol, the PFGE profiles produced were comparable between each centre. In most cases, there was at least 90% similarity between isolates tested in the different European laboratories and there was usually >95% similarity. This suggests that PFGE analyses are reproducible and therefore can be used as a valuable investigation tool combined with epidemiological data.Keywords
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