Studies on two Gibberellin-like Substances in Young Shoots of Tomato (Lycopersicon esculentumMill.)

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Abstract
Two gibberellin-like substances were found in the acidic fraction of shoot extracts of the tomato (Lycopersicon esculentum Mill., cultivar Potentate). These were resolved by paper chromotography with iso-propanol/ammonia/water (10:1:1) as the developing solvent but not with n-butanol/1.5 N ammonia (3:1). Both substances were active in the dwarf maize bioassay on mutants d-1, d-2, d-3, and d-5, and appeared to be more active on d-5 than d-1. Neither was active in the Meteor Pea assay. Neutral and basic fractions were inactive. The relative amounts of these two substances varied according to the age of the tissues from which they were extracted and this feature is discussed in relation to future studies on the physiology of gibberellin-like substances in vivo.