Practical Aspects of LC/EC Determinations of Pharmaceuticals in Biological Media

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Abstract
Liquid chromatography with electrochemical detection (LC/EC) has proven itself to be a very useful technique for the determination of electrochemically oxidizable or reducible compounds in complex matrices. Practical aspects of the application of LC/EC to pharmaceuticals in biological samples are discussed. These have been gleaned from several years experience with the determination of over a dozen compounds. The aspects discussed are a comparison of EC and UV detection which facilitates a choice between the two, and common problems including electrode coating, late eluters, detector temperature dependence and baseline instability. Various possible solutions to these problems are considered.