REPRESSED AND ACTIVE CHROMATIN ISOLATED FROM INTERPHASE LYMPHOCYTES

Abstract
Repressed and active chromatin can be isolated in mass from interphase calf thymus lymphocytes. Up to 80% of interphase lymphocyte DNA is found in the repressed chromatin fraction. Repressed interphase DNA appears as condensed (hetero-)chromatin, while active interphase DNA appears as extended (eu-)chromatin fibrils. The extended (eu-) chromatin fraction contains the bulk of the rapidly labeled RNA of the nucleus.

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