Interaction of oligoribocytidylates with T7 DNA in neutral and acid media

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Abstract
Oligoribocytidylates of chain length 4-12 interacted with native [phage] T7 DNA at neutral and slightly acid pH. Binding probably occurred at deoxycytosine clusters which may be displaced by the oligomers at neutral pH, while a local triple-stranded structure would be formed at acid pH. Transcription of DNA-(Cp)n complexes by Escherichia coli RNA polymerase showed a decrease in level without affecting the specificity of the transcription, suggesting that oligocytidylate binding did not occur on the promoters.

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