Functional Significance of the Specific Sites Phosphorylated in Desmin at Cleavage Furrow: Aurora-B May Phosphorylate and Regulate Type III Intermediate Filaments during Cytokinesis Coordinatedly with Rho-kinase
Open Access
- 1 April 2003
- journal article
- Published by American Society for Cell Biology (ASCB) in Molecular Biology of the Cell
- Vol. 14 (4) , 1489-1500
- https://doi.org/10.1091/mbc.e02-09-0612
Abstract
Aurora-B is a protein kinase required for chromosome segregation and the progression of cytokinesis during the cell cycle. We report here that Aurora-B phosphorylates GFAP and desmin in vitro, and this phosphorylation leads to a reduction in filament forming ability. The sites phosphorylated by Aurora-B; Thr-7/Ser-13/Ser-38 of GFAP, and Thr-16 of desmin are common with those related to Rho-associated kinase (Rho-kinase), which has been reported to phosphorylate GFAP and desmin at cleavage furrow during cytokinesis. We identified Ser-59 of desmin to be a specific site phosphorylated by Aurora-B in vitro. Use of an antibody that specifically recognized desmin phosphorylated at Ser-59 led to the finding that the site is also phosphorylated specifically at the cleavage furrow during cytokinesis in Saos-2 cells. Desmin mutants, in which in vitro phosphorylation sites by Aurora-B and/or Rho-kinase are changed to Ala or Gly, cause dramatic defects in filament separation between daughter cells in cytokinesis. The results presented here suggest the possibility that Aurora-B may regulate cleavage furrow-specific phosphorylation and segregation of type III IFs coordinatedly with Rho-kinase during cytokinesis.Keywords
This publication has 49 references indexed in Scilit:
- Phosphorylation of the Carboxyl Terminus of Inner Centromere Protein (INCENP) by the Aurora B Kinase Stimulates Aurora B Kinase ActivityJournal of Biological Chemistry, 2002
- Probing the Dynamics and Functions of Aurora B Kinase in Living Cells during Mitosis and CytokinesisMolecular Biology of the Cell, 2002
- Central Spindle Assembly and Cytokinesis Require a Kinesin-like Protein/RhoGAP Complex with Microtubule Bundling ActivityDevelopmental Cell, 2002
- Protein kinases required for segregation of vimentin filaments in mitotic processOncogene, 2001
- The Xenopus laevis Aurora/Ip11p-Related Kinase pEg2 Participates in the Stability of the Bipolar Mitotic SpindleExperimental Cell Research, 2000
- Rho-Associated Kinase Phosphorylates Desmin, the Myogenic Intermediate Filament Protein, at Unique Amino-Terminal SitesBiochemical and Biophysical Research Communications, 1998
- Roles of Rho-associated Kinase in Cytokinesis; Mutations in Rho-associated Kinase Phosphorylation Sites Impair Cytokinetic Segregation of Glial FilamentsThe Journal of cell biology, 1998
- A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancersThe EMBO Journal, 1998
- INTERMEDIATE FILAMENTS: Structure, Dynamics, Function and DiseaseAnnual Review of Biochemistry, 1994
- Sequence analysis of phosphoserine‐containing peptidesFEBS Letters, 1986