Isolated ε subunit ofBacillus subtilisF1-ATPase binds ATP
- 1 December 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (30) , 6875-6878
- https://doi.org/10.1016/j.febslet.2005.11.036
Abstract
Previously, we demonstrated ATP binding to the isolated ε subunit of F1-ATPase from thermophilic Bacillus PS3 [Kato-Yamada Y., Yoshida M. (2003) J. Biol. Chem. 278, 36013]. However, whether it is a general feature of the ε subunit from other sources is yet unclear. Here, using a sensitive method to detect weak interactions between fluorescently labeled ε subunit and nucleotide, it was shown that the ε subunit of F1-ATPase from Bacillus subtilis also bound ATP. The dissociation constant for ATP binding at room temperature was calculated to be 2mM, which may be suitable for sensing cellular ATP concentration in vivoKeywords
This publication has 23 references indexed in Scilit:
- ATP synthase — a marvellous rotary engine of the cellNature Reviews Molecular Cell Biology, 2001
- Crystal structure of the ϵ subunit of the proton-translocating ATP synthase from Escherichia coliStructure, 1997
- THE ATP SYNTHASE—A SPLENDID MOLECULAR MACHINEAnnual Review of Biochemistry, 1997
- The Trapping of Different Conformations of the Escherichia coli F1 ATPase by Disulfide Bond FormationJournal of Biological Chemistry, 1996
- Nucleotide-dependent Movement of the ε Subunit between α and β Subunits in the Escherichia coli F1F0-type ATPasePublished by Elsevier ,1996
- Structural features of the ε subunit of the Escherichia coli ATP synthase determined by NMR spectroscopyNature Structural & Molecular Biology, 1995
- Structure at 2.8 Â resolution of F1-ATPase from bovine heart mitochondriaNature, 1994
- Characterization of the inhibitory (.epsilon.) subunit of the proton-translocating adenosine triphosphatase from Escherichia coliBiochemistry, 1980
- Inhibitory properties of endogenous subunit ϵ in the Escherichia coli F1 ATPaseArchives of Biochemistry and Biophysics, 1979
- Partial purification of active delta and epsilon subunits of the membrane ATPase from escherichia coliJournal of Supramolecular Structure, 1975