Enzyme Immunoassay and Two Fluorometric Methods Compared for the Determination of Quinidine in Serum

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Abstract
Quinidine, an antiarrhythmic drug, was quantitated in serum by a commercially supplied enzyme immunoassay procedure (EMIT). Replicate analyses of serum controls resulted in a within-assay coefficient of variation of .ltoreq. 10% and a between-assay coefficient of variation of .ltoreq. 7%. Patient quinidine results by enzyme immunoassay were compared to those obtained by a nonselective (protein precipitation) and a selective (solvent back-extraction) fluorometric method. Assay selectivity for quinidine vs. other drugs and vs. the 3-OH metabolite of quinidine was determined. The enzyme immunoassay technique is sensitive for quinidine and more selective than commonly used fluorometric methods relying on back-extraction or protein precipitation.

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