Acetylation of prostaglandin synthetase by aspirin. Purification and properties of the acetylated protein from sheep vesicular gland

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Abstract
Aspirin (acetylsalicylic acid) [an anti-inflammatory agent] apparently inhibits prostaglandin synthetase by acetylating an active site of the enzyme. The enzyme was labeled from an acetone-pentane powder of sheep vesicular gland using [acetyl-3H]aspirin and purified the [3H]-acetyl-protein to near homogeneity. The final preparation contains protein of a single MW (85,000) and an amino-terminal sequence of Asp-Ala-Gly-Arg-Ala. The [3H]-acetyl-protein contained 0.5 mol of acetyl residues per mol of protein based on amino acid composition, but only a single sequence was found.