Growth of Pathogenic Leptospira in Chemically Defined Media

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Abstract
A protein-free chemically defined medium for cultivation of pathogenic Leptospira was developed. The medium permitted continued serial subculturing of 9 serogroups (52 strains) of the 12 serogroups (61 strains) tested. Growth was initiated from small inocula, and the growth rate and maximal cell yields were similar to those on serum-containing media. The nutritional requirements of serogroups L. canicola, L. pomona , and L. grippotyphosa were studied in a basal medium composed of inorganic salts, a fatty acid, vitamin B 12 , and thiamine. All strains tested utilized ammonium chloride as the sole nitrogen source. A fatty acid, vitamin B 12 , and ferrous ions were essential. Growth was stimulated by thiamine, potassium, and calcium ions.