DNA sequence coding for an antifreeze protein precursor from winter flounder.
- 1 January 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (2) , 335-339
- https://doi.org/10.1073/pnas.79.2.335
Abstract
A c[complementary]DNA made to antifreeze protein mRNA of the winter flounder was cloned in the plasmid pBR322 and its sequence was determined by the method of Maxam and Gilbert. Its sequence codes for a precursor protein that is 82 amino acids in length. This precursor has both a signal polypeptide and a pro-sequence before the mature protein of 38 amino acid residues. The mature protein matches in composition 1 of the alanine-rich serum antifreeze proteins that was purified by ion-exchange and reverse-phase chromatography. The composition of the pro-sequence is similar to that of the native protein except that it contains 5 prolines. The mature protein, but not the pro-sequence, contains 3 of the 11-residue repeats previously observed in 2 other antifreeze protein components.This publication has 17 references indexed in Scilit:
- Precursor of egg white ovomucoid. Amino acid sequence of an NH2-terminal extension.Journal of Biological Chemistry, 1978
- In vivo biosynthesis of the antifreeze protein in the winter flounder — Evidence for a larger precursorBiochemical and Biophysical Research Communications, 1978
- Synthesis of double-stranded DNA complementary to lysozyme, ovomucoid, and ovalbumin mRNAs. Optimization for full length second strand synthesis by Escherichia coli DNA polymerase I.Journal of Biological Chemistry, 1978
- Structure of a peptide antifreeze and mechanism of adsorption to iceBiochimica et Biophysica Acta (BBA) - Protein Structure, 1977
- Circular dichroism of protein and glycoprotein fish antifreezesBiopolymers, 1977
- Circannual cycles of blood plasma freezing point and Na+ and Cl− concentrations in Newfoundland winter flounder (Pseudopleuronectes americanus): correlation with water temperature and photoperiodCanadian Journal of Zoology, 1977
- Structural studies on the freezing-point-depressing protein of the winter flounder Pseudopleuronectes americanusBiochemical and Biophysical Research Communications, 1977
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976
- The synthesis of freezing-point-depressing protein of the winter flounder Pseudopleuronectusamericanus in Xenpuslaevis oocytesBiochemical and Biophysical Research Communications, 1976
- Prediction of protein conformationBiochemistry, 1974