Evaluation of a diet dilution technique for measuring the response of broiler chickens to increasing concentrations of lysine

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Abstract
1. Three experiments were conducted on male broiler chickens between one and three weeks of age to determine their response to dietary lysine concentrations. 2. Serial dilutions of a summit diet shown to be first‐limiting in lysine were fed in all experiments. The balance between amino acids in these diets was maintained within narrow limits. 3. Intake of the most‐limiting amino acid was the most important factor determining growth rate; protein intake as such was of little or no importance. 4. The efficiency of utilisation of dietary lysine for protein growth was calculated to be 65–05 mg/g protein gain, representing a net efficiency of 0–85. 5. The diet dilution technique overcomes the major disadvantage of the graded supplementation method for determining the requirements of amino acids, namely that of the amino acid balance changing systematically in successive dietary treatments.