β-D-galactosidase activity of viable, non-culturable coliform bacteria in marine waters
Open Access
- 1 August 1995
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 21 (2) , 99-102
- https://doi.org/10.1111/j.1472-765x.1995.tb01016.x
Abstract
The β-D-galactosidase activity of viable but non-culturable (vnc) Escherichia coli cells in seawater was investigated using a rapid fluorimetric enzyme assay. Results from microcosm studies showed that loss of culturability did not necessarily result in loss of the ability to produce the galactosidase enzyme. Even when no culturable cells were detected, a positive enzyme assay response was observed and the activity of the inducible enzyme over time more closely reflected the number of vnc cells present.Keywords
This publication has 13 references indexed in Scilit:
- Possible interference of lactose‐fermenting marine vibrios in coliform ‐D‐galactosidase assaysJournal of Applied Bacteriology, 1995
- Plant and algal interference in bacterial beta-D-galactosidase and beta-D-glucuronidase assaysApplied and Environmental Microbiology, 1994
- Methionine uptake and cytopathogenicity of viable but nonculturable Shigella dysenteriae type 1Applied and Environmental Microbiology, 1994
- Monitoring of fecal pollution in coastal waters by use of rapid enzymatic techniquesApplied and Environmental Microbiology, 1994
- Rapid detection of sewage contamination in marine waters using a fluorimetric assay of β-d-galactosidase activityScience of The Total Environment, 1994
- The modification and evaluation of some cytochemical techniques for the enumeration of metabolically active heterotrophic bacteria in the aquatic environmentJournal of Applied Bacteriology, 1984
- Survival and viability of nonculturableEscherichia coli andVibrio cholerae in the estuarine and marine environmentMicrobial Ecology, 1982
- Use of nuclepore filters for counting bacteria by fluorescence microscopyApplied and Environmental Microbiology, 1977
- Studies on the induction of β-galactosidase in a cryptic strain of Escherichia coliBiochimica et Biophysica Acta, 1959