Characterization and differential expression of protein kinase C isoforms in PC12 cells Differentiation parallels an increase in PKC beta11
- 17 February 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 298 (1) , 74-78
- https://doi.org/10.1016/0014-5793(92)80025-c
Abstract
Nerve growth factor (NGF) treatment of PC12 cells induced a 2.8‐fold increase in protein kinase C activity concomitant with differentiation and acquisition or neurites. PKC protein isoforms were separated by sequential chromatography on DEAE‐Sephacel/hydroxylapatite. A broad peak or PKC activity eluted which corresponded to the alpha PKC isoform. In control cells, message for all six PKC isoforms was detected and expressed as epsilon>zeta=gamma>delta>beta>alpha. Western blot or whole cell lysates revealed a large increase in the beta11, while slight changes were observed for the other five PKC isoforms during treatment (12‐14 days) with NGF (50 ng/ml). In parallel, coordinate changes in the expression of the individual transcripts for the six isoforms occurred during NGF treatment. Induction and accumulation of PKC beta11 may play a role in maintenance or neuronal morphology.Keywords
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